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目的:探讨标本溶血对常规生化检验项目的影响。方法:选择余姚市中医医院门诊行健康体检的150例健康志愿者(2018年1－10月)进行前瞻性研究，在清晨采集健康志愿者空腹状态下的肘部静脉血液共10 mL作为检测标本，将每位健康志愿者的血液标本均分为2份，每份5 mL，分别设置为对照组标本、观察组标本，对照组血液标本未经溶血处理，直接进行生化检验，观察组血液标本经溶血处理后再进行生化检验，比较两组血液标本的常规生化检验项目检测结果。结果:观察组总蛋白(TP)、白蛋白(ALB)、球蛋白(G)、总胆红素(TBIL)、直接胆红素(DBIL)、谷氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、胆碱酯酶(CHE)分别为(71.49±1.62)g/L、(48.09±0.85)g/L、(25.18±0.67)g/L、(9.31±1.14)μmol/L、(1.89±0.34)μmol/L、(23.14±1.70)μmol/L、(27.38±2.56)μmol/L、(224.53±5.68)U/L，均高于对照组(n t＝9.351、9.235、8.786、9.161、9.309、8.877、9.391、9.878，均n P<0.05)。观察组血肌酐(Scr)、尿素氮(BUN)、尿酸(UA)分别为(87.93±2.76)μmol/L、(5.43±0.47)mmol/L、(249.38±7.52)μmol/L，均高于对照组(n t＝9.367、8.744、9.644，均n P<0.05)。观察组总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)分别为(4.53±0.49)mmol/L、(1.02±0.26)mmol/L、(1.92±0.40)mmol/L，均高于对照组(n t＝9.481、8.795、8.470，均n P<0.05)，其高密度脂蛋白胆固醇(HDL-C)(1.49±0.22)mmol/L，低于对照组(n t＝9.085，n P<0.05)。观察组的肌酸激酶(CK)、羟丁酸脱氢酶(HBD)、乳酸脱氢酶(LDH)分别为(41.27±1.85)U/L、(116.73±6.24)U/L、(198.94±6.07)U/L，均高于对照组(n t＝9.433、9.610、9.890，均n P<0.05)。观察组钠(Nan ＋)、钾(Kn ＋)、钙(Can 2＋)、氯(Cln －)分别为(142.31±2.17)mmol/L、(4.82±0.45)mmol/L、(2.49±0.09)mmol/L、(101.89±1.45)mmol/L，均高于对照组(n t＝7.412、9.040、8.593、8.359，均n P<0.05)。n 结论:血液标本发生溶血反应后会对常规生化检验项目的检测结果产生影响，易导致生化检验结果误差。“,”Objective:To study the effect of hemolysis on routine biochemical test items.Methods:From January 2018 to October 2018, 150 healthy volunteers who underwent physical examination in the outpatient department of Yuyao Hospital of Traditional Chinese Medicine were selected in this prospective study.In the early morning, 10 mL blood samples of elbow vein were collected from healthy volunteers on an empty stomach.The blood samples of each healthy volunteer were divided into two parts, with 5 mL in each sample, and were set up as control group and observation group respectively.The blood samples of the control group were directly conducted biochemical test without hemolysis treatment.The blood samples of the observation group were biochemical tested after hemolysis treatment.The results of routine biochemical test items of the blood samples of the two groups were compared.Results:In the observation group, the TP, Alb, G, TBIL, DBIL, ALT, AST and CHE were (71.49±1.62)g/L, (48.09±0.85)g/L, (25.18±0.67)g/L, (9.31±1.14)μmol/L, (1.89±0.34)μmol/L, (23.14±1.70)μmol/L, (27.38±2.56)μmol/L, (224.53±5.68)U/L, respectively, which were higher than those in the control group (n t=9.351, 9.235, 8.786, 9.161, 9.309, 8.877, 9.391, 9.878, all n P<0.05). The Scr, BUN and UA of the observation group were (87.93±2.76)μmol/L, (5.43±0.47)μmol/L, (249.38±7.52)μmol/L, respectively, which were higher than those of the control group (n t=9.367, 8.744, 9.644, all n P<0.05). The TC, TG and LDL-C in the observation group were (4.53±0.49)mmol/L, (1.02±0.26)mmol/L, (1.92±0.40)mmol/L, respectively, which were higher than those in the control group (n t=9.481, 8.795, 8.470, all n P<0.05), and the HDL-C in the observation group[(1.49±0.22)mmol/L]was lower than that in the control group (n t=9.085, n P<0.05). The CK, HBD and LDH in the observation group were (41.27±1.85)U/L, (116.73±6.24)U/L and (198.94±6.07) U/L, respectively, which were higher than those in the control group (n t=9.433, 9.610, 9.890, all n P<0.05). The Nan + , Kn + , Can 2+ , and Cln - of the observation group were (142.31±2.17)mmol/L, (4.82±0.45)mmol/L, (2.49±0.09)mmol/L, (101.89±1.45)mmol/L, respectively, which were higher than those of the control group (n t=7.412, 9.040, 8.593, 8.359, all n P<0.05).n Conclusion:Hemolysis reaction of blood sample will affect the results of routine biochemical test items, which will easily lead to the error of biochemical test results.