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本文采用小鼠骨髓和人淋巴细胞微核试验方法测试了新疆产的红花的诱变性。结果表明,红花水提取物和乙醚提取物均能诱发小鼠骨髓嗜多染红细胞(PCE)微核率升高。红花水提取物的剂量在4～20ml/kg体重范围内,乙醚提取物剂量在1～4ml/kg体重诱发的小鼠骨髓PCE微核率分别为5.16±0.83‰～8.33±0.76‰,3.83±0.48～5.17±0.83‰,与阴性对照(2.5±0.34‰)比较,差异极显著(P<0.01)。红花水提取物亦能诱发人淋巴细胞微核率升高,在实验范围内(0.5～1.5μl/ml)诱发的微核率(3‰～6‰)与阴性对照(2‰)比较有显著性差异(P<0.05)。
In this paper, the mutagenicity of safflower produced in Xinjiang was tested using mouse bone marrow and human lymphocyte micronucleus test methods. The results showed that both the safflower water extract and the ether extract can induce the increase of the micronucleus rate of mouse bone marrow polychromatic erythrocytes (PCE). The dose of safflower water extract was in the range of 4-20 ml/kg body weight, and the micronucleus rate of PCE in mouse bone marrow induced by the ether extract dose of 1-4 ml/kg body weight was 5.16±0.83 ‰8.33±0.76 ‰, 3.83, respectively. ± 0.48 ~ 5.17 ± 0.83 ‰, compared with the negative control (2.5 ± 0.34 ‰), the difference was extremely significant (P <0.01). The safflower water extract also induces an increase in the micronucleus rate of human lymphocytes. The micronucleus rate (3‰ to 6‰) induced in the experimental range (0.5～1.5μl/ml) is comparable to the negative control (2‰). Significant difference (P<0.05).