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A hollow-fiber liquid-phase microextraction(HF-LPME) method has been developed for the purification and preconcentration of biogenic polyamines and their precursor amino acids in human saliva.Putrescine(Put),cadaverine(Cad),spermidine(Spe),ornithine(Orn),lysine(Lys),and arginine(Arg)were determined by the CE-LIF detection after microextraction.Several factors that affect extraction efficiency,separation,and detection were investigated.Under the optimum conditions,six analytes could achieve baseline separation within 30 min,exhibiting a linear calibration at three orders of magnitude(r~2 > 0.998);the obtained enrichment factors of HF-LPME were between 19(for Orn) and 218(for Cad),and the LODs were in the range of 0.0072-0.26 nmol/L.The proposed HF-LPME/CE-LIF method has been successfully applied for the sensitive analyses of the real-world saliva samples collected from healthy volunteers and different patients with oral diseases,providing a potential method for primary non-invasive diagnosis of some oral diseases.
A hollow-fiber liquid-phase microextraction (HF-LPME) method has been developed for the purification and preconcentration of biogenic polyamines and their precursor amino acids in human saliva. Putrescine (Put), cadaverine (Cad), spermidine (Spe), ornithine (Orn), lysine (Lys), and arginine (Arg) were determined by the CE-LIF detection after microextraction. Serral factors that affect extraction efficiency, separation, and detection were investigated. Undertaking the optimum conditions, six analytes could achieve baseline separation within 30 min, exhibiting a linear calibration at three orders of magnitude (r ~ 2> 0.998); the obtained enrichment factors of HF-LPME were between 19 (for Orn) and 218 (for Cad), and the LODs were in the range of 0.0072-0.26 nmol / L.The proposed HF-LPME / CE-LIF method has been successfully applied for the sensitive analyzes of the real-world saliva samples collected from healthy volunteers and different patients with oral diseases, providing a potential method for primary non-invasive diag nosis of some oral diseases.