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目的:研究灵仙新苷(clematichinenoside AR)调节血脂及抗动脉粥样硬化的作用。方法:采用高脂饲料喂饲和腹腔注射维生素D3结合腹腔注射LPS建立大鼠动脉粥样硬化模型,造模成功后灌胃给予高、中、低剂量的AR(32,16,8 mg.kg-1.d-1),8周后测定大鼠血清胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白-胆固醇(HDL-C)、低密度脂蛋白-胆固醇(LDL-C)、肿瘤坏死因子(TNF-α)、一氧化氮(NO)及诱导型一氧化氮合酶(iNOS);取其胸主动脉进行油红O染色,观察大鼠胸主动脉内膜脂质沉积情况;取肝脏进行常规HE染色,观察肝脏病变。结果:AR高、中剂量组能显著降低高血脂症大鼠血清TC,TG,LDL-C,TNF-α,NO水平和iNOS活力,升高HDL-C水平,差异具有统计学意义(P<0.05)。同时,AR能减少主动脉内皮脂质沉积,改善肝脏的脂肪变性和炎症细胞浸润。结论:AR具有调节血脂,减轻动脉粥样硬化发生和发展的作用,该作用与其调节NO及其合酶的形成和表达相关。
Objective: To study the effect of clematichinenoside AR in regulating blood lipid and anti-atherosclerosis. Methods: The model of atherosclerosis was established by intraperitoneal injection of high fat diet and intraperitoneal injection of vitamin D3 and LPS. The rats were given intragastric administration of high, medium and low doses of AR (32, 16, and 8 mg.kg The levels of serum TC, TG, HDL-C and LDL-C in rats were measured after 8 weeks. , Tumor necrosis factor (TNF-α), nitric oxide (NO) and inducible nitric oxide synthase (iNOS). The thoracic aorta was taken for oil red O staining to observe the intimal lipid deposition Condition; take the liver routine HE staining, observe the liver lesions. Results: The levels of TC, TG, LDL-C, TNF-α, NO and iNOS in hyperlipidemic rats were significantly decreased and the levels of HDL-C were significantly increased in AR high and middle dose groups (P < 0.05). At the same time, AR can reduce aortic endothelial lipid deposition, improve liver steatosis and inflammatory cell infiltration. Conclusion: AR can regulate blood lipids and alleviate the occurrence and development of atherosclerosis, which is related to the regulation of NO and its synthase formation and expression.