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目的:研究重组人粒细胞集落刺激因子(recombinant human granulocyte colony-stimulating factor,rhG-CSF)对大鼠局灶性脑缺血的神经元保护作用。方法:用线栓法建立大鼠大脑中动脉缺血再灌注模型,于再灌注不同时间点对治疗组和对照组进行神经缺损评分(NSS);并用免疫组织化学方法测定神经上皮干细胞蛋白(nestin)的表达。结果:与对照组相比,rhC-CSF治疗组大鼠的神经缺损评分明显降低(P<0.05);治疗组脑梗死区周围的nestin表达较对照组增多。结论:脑缺血再灌注后,一定剂量rhG-CSF可能通过提高nestin的表达,增加神经细胞的修复,改善脑卒中的预后。
Objective: To study the neuroprotective effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on focal cerebral ischemia in rats. Methods: The model of middle cerebral artery occlusion (MCAO) was established by thread occlusion. The nerve deficit score (NSS) was measured at different time points after reperfusion. The expression of nestin )expression. Results: Compared with the control group, the score of neurological deficit in rhC-CSF treatment group was significantly lower (P <0.05). The expression of nestin around the infarct zone in the treatment group was higher than that in the control group. Conclusion: After cerebral ischemia and reperfusion, rhG-CSF may increase the expression of nestin, increase the repair of nerve cells and improve the prognosis of stroke.