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Functional Ni2+-polyacrylic acid (Ni2+-PAA) adsorbent has been prepared for metal chelate affinity chromatography. DNA elements coding for adjacent histidines were fused to the Aeromonas hydrophila ompTS gene. Subsequent expression in E. coli resulted in the production of hybrid protein 6His-OmpTS that could be purified by Ni2+-PAA affinity chromatography.
Functional analysis of Ni2 + -polyacrylic acid (Ni2 + -PAA) adsorbent has been prepared for metal chelate affinity chromatography. DNA elements coding for adjacent histidines were fused to the Aeromonas hydrophila ompTS gene. Subsequent expression in E. coli resulted in the production of hybrid protein 6His- OmpTS that could be purified by Ni2 + -PAA affinity chromatography.