Biochar has been widely applied for the remediation of petroleum-contaminated soil.How-ever,the effect of biochar on the transport of petroleum degradation bacteria has not been studied.A typical Gram-positive petroleum degradation bacteria-Corynebacterium uariabile HRJ4 was used to study the effect of different biochars on bacterial transport and reten-tion.Results indicated that the addition of biochar in sand was effective for reducing the transport of bacteria and poplar sawdust biochar(PSBC)had a stronger hinder effect than corn straw biochar(CSBC).The hindrance was more evident with pyrolysis temperature of biochar raised from 300℃ to 600℃,which was attributed to the increase of specific surface area(309 times).The hindrance effect also enhanced with higher application rate of biochar.Furthermore,the reduction of HRJ4 transport was more obvious in higher(25 mmol/L)con-centration of NaCl solution owing to electrostatic attraction enhancement.The adsorption of biochar to HRJ4 was defined to contribute to the hindrance of HRJ4 transport mainly.Combining the influence of feedstocks and pyrolysis temperature on HRJ4 transport,it sug-gested that specific surface area had the greatest effect on HRJ4 transport,and pore-filling,electrostatic force also contributed to HRJ4 retained in quartz sand column.At last,phenol transportation experiment indicated that the restriction of biochar on HRJ4 enhanced the phenol removal rate in the column.This study provides a theoretical basis for the interac-tion of biochar and bacteria,which is vital for the remediation of oil-contaminated soil and groundwater in the field.