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目的探讨阿魏酸衍生物对心肌细胞缺氧复氧(H/R)损伤的影响。方法用1~3d的SD大鼠进行心肌细胞原代培养,取生长5~6d的心肌细胞分为对照组、H/R组、溶剂组、不同阿魏酸衍生物组,除对照组外其他组均做H/R模型。通过测定肌酸激酶(CK)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)及丙二醛(MDA)的变化,观察不同阿魏酸衍生物对心肌细胞H/R损伤的保护作用。结果与H/R比较,除IIa外,阿魏酸类似物均可明显减少心肌细胞H/R损伤所造成的CK、LDH的渗出、MDA含量,同时可增加SOD酶活力。结论阿魏酸衍生物苯环上3,5位取代基可以明显影响此类化合物抗氧化活性,甲氧基和乙氧基表现为提高活性,溴次之。
Objective To investigate the effect of ferulic acid derivatives on cardiomyocyte hypoxia-reoxygenation (H / R) injury. Methods Primary cardiomyocytes were cultured with 1 ~ 3d SD rats. Cardiomyocytes cultured for 5 ~ 6 days were divided into control group, H / R group, solvent group and different ferulic acid derivatives group, except the control group Group are done H / R model. The changes of creatine kinase (CK), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured to observe the effect of different ferulic acid derivatives on H / R injury of cardiomyocytes Protective effects. Results Compared with H / R, except for IIa, ferulic acid analogues could significantly reduce the exudation of CK and LDH, MDA content caused by H / R injury of cardiomyocytes, and increase the activity of SOD. Conclusion The 3,5-substituent on the benzene ring of ferulic acid derivatives can obviously affect the anti-oxidative activity of these compounds. The methoxy and ethoxy groups show the activity of bromine and bromine.