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自南京及西安各医院分离的金黄色葡萄球菌中通过质粒消除试验、转导及原生质体转化进行了抗生素抗药基因的定位。实验证明在２３株菌中，编码青霉素、四环素、氯霉素及红霉素抗药性的基因存在４２个抗药质粒上，编码青霉素、四环素、红霉素、卡那霉素、庆大霉素及链霉素的抗药基因存在染色体上。提供了含上述抗药质粒的质粒谱。青霉素抗药质粒的分子量为１１.６～１８.４Ｍｄ，四环素抗药质粒为２.８～４.２Ｍｄ，氯霉素抗药质粒为１.８～２.０Ｍｄ，红霉素抗药质粒为１.５～２.５Ｍｄ。从４株代表菌中通过原生质体转化或转导获得９株含单一抗药质粒的克隆，它们所含质粒的大小及抗药水平也已加测定。
Antibiotic resistance genes were located in S. aureus isolated from Nanjing and Xi’an hospitals by plasmid abatement assays, transduction and protoplast transformation. Experiments show that in 23 strains of bacteria, the genes encoding penicillin, tetracycline, chloramphenicol and erythromycin resistance exist 42 drug-resistant plasmids encoding penicillin, tetracycline, erythromycin, kanamycin, gentamicin And streptomycin resistant genes exist on the chromosome. Plasmid profiles containing the above drug-resistant plasmids are provided. The molecular weight of the penicillin-resistant plasmid was 11.6-18.4Md, that of tetracycline-resistant plasmid was 2.8-4.2Md, that of chloramphenicol-resistant plasmid was 1.8-2.0Md, erythromycin-resistant plasmid was 1.5 ~ 2.5Md. Nine strains with single drug-resistant plasmids were obtained by transformation or transduction of protoplasts from four representative strains. The size of plasmids contained and the drug resistance levels were also determined.