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我国是第一大豌豆生产国,在世界豌豆生产中占有举足轻重的地位。然而,干旱是制约我国豌豆生产的主要因素,为了探索豌豆芽期耐旱性,以4份来自不同降雨量地区的豌豆种质为材料,在浓度为0、5%、10%、15%、20%、25%和30%PEG-6000(聚乙二醇)高渗溶液模拟干旱条件产生水分胁迫进行种子萌发试验,试验中测定种子的发芽势、发芽率、发芽指数及简易活力指数4个指标的动态变化,确定了豌豆芽期耐旱性鉴定适宜的PEG-6000浓度为20%~25%。进一步以22%PEG-6000模拟旱胁迫对来自我国18个不同省份的87份豌豆种质资源进行耐旱性鉴定,试验中测定种子相对发芽势、相对发芽率等、萌发耐旱指数及萌发胁迫指数等13个指标,主成分分析确定了相对发芽势、相对发芽率等7项指标作为耐旱综合评价因子,利用隶属函数法,筛选出1份高抗种质(G0002457),来自降水较少的新疆莎车,7份抗旱种质(G0002293、G0005403、G0002418、G0002083、G0000799、G0002017和G0002082),多来自降水较少的西北部春播区。本研究可为耐旱种质的选择提供科学指导,为豌豆耐旱品种改良奠定基础。
China is the largest producer of pea and occupies a pivotal position in the world pea production. However, drought is the main factor restricting the pea production in China. In order to explore the drought-resistance of pea budding period, four pea germplasms from different rainfall areas were used as material, The seed germination test was conducted under 20%, 25% and 30% PEG-6000 (polyethylene glycol) hypertonic solution to simulate the drought conditions to produce water stress. The germination potential, germination rate, germination index and simple vigor index The dynamic changes of the indexes indicated that the optimal PEG-6000 concentration for identifying the drought-tolerance of pea buds was 20% -25%. The drought resistance of 87 pea germplasm resources from 18 different provinces in China were identified by drought stress with 22% PEG-6000. The relative germination potential, relative germination rate, germination drought tolerance index and germination stress Index and other 13 indicators, the principal component analysis to determine the relative germination potential, the relative germination rate of seven indicators as a comprehensive evaluation of drought resistance factors using membership function, screening out 1 high germplasm (G0002457), from less precipitation Xinjiang Shache, 7 drought-resistant germplasm (G0002293, G0005403, G0002418, G0002083, G0000799, G0002017 and G0002082), mostly from the spring precipitation area of northwestern China with less precipitation. This study can provide scientific guidance for the selection of drought-resistant germplasm and lay a foundation for the improvement of drought-tolerant pea varieties.