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目的探讨结核菌株进行多位点可变数目串联重复序列分析(the multiple locus VNTR analysis,MLVA)。方法选择2010年至2011年新疆维吾尔自治区第五次结核病流行病学抽样调查资料,采用经典24位点方法进行基因分型。并采用Bio Numerics5.0数据库进行基因聚类分析。将结核分枝杆菌原始株,取一菌环溶于400μl TE中悬菌,80℃1 h灭活,12 000 r/min离心10 min,弃上清,600μl TE重新悬菌,进行多位点串联重复序列分析。结果新疆99株结核分枝杆菌分为2个基因群:分别为基因群Ⅰ和基因群Ⅱ,基因群Ⅰ66株(66.7%),基因株Ⅱ33株(33.3%);基因群Ⅰ是北京家族,基因群Ⅰ的66株结核分枝杆菌有65种不同的基因型,有2株结核分枝杆菌属于同一簇,成簇率为1.5%,基因群II33株结核分枝杆菌菌株的MLVA图谱不同,成簇率为0。结论新疆结核分枝杆菌菌株存在明显的基因多态性,以北京基因型菌株为主,同时还存在一定比例的非北京基因型,应加强对主要流行菌株流行的监控及管理。
Objective To investigate the multiple locus VNTR analysis (MLVA) of tuberculosis strains. Methods The data of the fifth epidemiological survey of tuberculosis in Xinjiang Uygur Autonomous Region from 2010 to 2011 were selected and genotyped by classical 24-locus method. Bio Numerics5.0 database was used for gene cluster analysis. The Mycobacterium tuberculosis original strain, take a loop dissolved in 400μl TE suspension, inactivated 1 h at 80 ℃, centrifuged at 12 000 r / min 10 min, the supernatant was discarded, 600μl TE resuspended, multi-site Tandem repeat analysis. Results 99 strains of Mycobacterium tuberculosis in Xinjiang were divided into two groups: gene group Ⅰ and group Ⅱ, group Ⅰ66 (66.7%) and group Ⅱ33 (33.3%). Group Ⅰ was a member of Beijing family, Mycobacterium tuberculosis 66 strains of genotype Ⅰ 65 different genotypes, two strains of Mycobacterium tuberculosis belong to the same cluster, the clustering rate was 1.5%, the genotype group II33 strains of Mycobacterium tuberculosis MLVA profiles are different, The clustering rate is zero. Conclusion Mycobacterium tuberculosis strains in Xinjiang have obvious genetic polymorphisms, mainly Beijing genotype strains, and a certain proportion of non-Beijing genotypes exist at the same time. Therefore, the surveillance and management of the prevalence of major epidemic strains should be strengthened.