将0.4mM的槲皮甙与0.5g新鲜的人粪便(保存于充满无氧二氧化碳的维尼龙袋中)混合,于37℃进行厌氧培养20h,然后用乙酸乙酯提取培养物3次,提取物用硅胶柱层析分离,用氯仿-甲醇(5:1)作为洗脱剂。主要洗脱产物用甲醇结晶,通过红外、紫外、核磁、质谱鉴定其为槲皮素。其他苯酚类洗脱产物均用TLC进 0.4 mM quercitrin was mixed with 0.5 g of fresh human feces (preserved in a vinylon bag filled with anaerobic carbon dioxide) and incubated at 37°C for 20 hours. After that, the culture was extracted 3 times with ethyl acetate and extracted. The material was separated using silica gel column chromatography with chloroform-methanol (5:1) as eluent. The main eluted product was crystallized from methanol and identified as quercetin by infrared, ultraviolet, nuclear magnetic, and mass spectrometry. Other phenol products eluted with TLC