目的　为探讨铝的发育毒性及机理。方法孕 9.5d大鼠胚胎于体外培养系统中给予不同剂量的硫酸铝 ,培养 4 8h后 ,观察胚胎生长发育和器官形态分化状况 ;应用二硫代双硝基苯甲酸 (DTNB)直接法测定胚胎组织谷胱甘肽 (GSH)含量 ;以 1,6 二苯己三烯为荧光探剂 ,用荧光偏振技术测定卵黄囊细胞膜脂质流动性。结果　当培养液中铝浓度为1.2mg·L- 1时 ,胚胎生长发育和分化明显被抑制 ;3.0mg·L- 1时 ,畸形胚胎发生率明显升高 ,主要有神经管闭合不全 ,脑发育不良和体翻转不全 ;6 .0mg·L- 1时 ,胚胎组织GSH含量和卵黄囊细胞膜脂质流动性显著降低。上述效应均呈现出一定的剂量 效应 (反应 )关系。结论　铝有潜在的致畸性和胚胎毒性 ,胚胎组织GSH含量和卵黄囊细胞膜流动性降低可能在铝致胚胎发育毒性中起重要作用 Objective To investigate the developmental toxicity and mechanism of aluminum. Methods Different doses of aluminum sulfate were administered to rat embryos cultured in vitro for 9.5 days. After 48 h incubation, the embryonic development and morphological differentiation of the embryos were observed. The embryos were determined by direct DTNB assay The content of glutathione (GSH) was measured. Fluorescence polarization was used to determine the lipid fluidity of yolk sac cell membrane with 1,6 diphenylhexane as fluorescent probe. Results When the concentration of aluminum in the culture medium was 1.2 mg · L -1, the growth and differentiation of embryos were obviously inhibited. When the concentration of aluminum was 3.0 mg · L -1, the incidence of abnormal embryos was significantly increased. The main defects were neural tube insufficiency and brain development Poor and incomplete body turnover; 6 .0mg · L -1, embryonic tissue GSH content and yolk sac cell membrane lipid fluidity was significantly reduced. The above effects show a certain dose-response (response) relationship. Conclusion Aluminum has potential teratogenicity and embryotoxicity. The reduction of GSH content in embryonic tissues and the fluidity of yolk sac cell membrane may play an important role in the developmental toxicity of aluminum to embryogenesis