Synthesis of Cell Adhesive Motif RGD Tripeptide by a Novel Chemical Method and Its Purification

来源 :Chemical Research in Chinese Universities | 被引量 : 0次 | 上传用户:crowboy2000
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The cell adhesive motif RGD tripeptide was synthesized by using a novel chemical method. First, Gly-Asp(GD) was synthesized in two steps including the chloroacetylation of free L-aspartic acid and the ammonolysis of the chloroacetylated L-aspartic acid. The yield of chloroacetylated L-aspartic acid was 83.0%. For the ammonolysis of chloroacetylated L-aspartic acid, the yield of the ammonolyzed product was 92.3%. Second, the coupling between Arg and Gly-Asp was carried out by using the NCA method. The maximum yield of RGD was about 50% at 0 ℃ and pH=9.5. The prepared RGD tripeptide was confirmed by using amino acid component analysis and mass spectrographic {analysis.} The cell adhesive motif RGD tripeptide was synthesized by using a novel chemical method. First, Gly-Asp (GD) was synthesized in two steps including the chloroacetylation of free L-aspartic acid and the ammonolysis of the chloroacetylated L-aspartic acid. The yield of the chloroacetylated L-aspartic acid was 83.0%. For the ammonolysis of chloroacetylated L-aspartic acid, the yield of the ammoniated product was 92.3%. Second, the coupling between Arg and Gly-Asp was carried out by using the NCA method. The The maximum yield of RGD was about 50% at 0 ° C and pH = 9.5. The prepared RGD tripeptide was confirmed by using amino acid component analysis and mass spectrographic {{analysis. }
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