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目的:探讨高频雾化吸入茶黄素乳酸-羟基乙酸共聚物(poly lactic-co-glycolic acid,PLGA)纳米粒对炎性气道黏蛋白5AC(mucoprotein 5AC,MUC5AC)的作用。方法:通过改良法制备茶黄素PLGA纳米粒,用粒径分析仪检测纳米粒平均粒径,并进行包封率和载药量测定及体外释放试验。将SD大鼠分为3组:对照组(A组)、香烟吸入组(B组)及干预组(C组)。RT-PCR检测各组黏蛋白5AC m RNA含量;酶联免疫吸附测定法(ELISA)观察MUC5AC蛋白表达的变化,并进行组化对比大鼠气道组织黏蛋白变化情况。结果:制备的茶黄素PLGA纳米粒性质稳定,测得其的平均粒径为(108.24±2.86)nm,适宜雾化吸入。香烟吸入组MUC5AC的蛋白表达明显升高,较对照组有显著性差异(P=0.000),而给予茶黄素PLGA纳米粒处理后,与香烟组比,指标明显下调(P=0.001)。MUC5AC m RNA表达的RT-PCR电泳结果也有类似结果。气道组织化学PAS染色也提示雾化吸入茶黄素PLGA纳米粒后,气道表面黏蛋白表达显著下降。结论:通过改良法制备茶黄素PLGA纳米粒性质稳定,大小均匀;经雾化途径吸入可发挥抑制炎性气道的黏蛋白5AC的高分泌作用,为茶黄素的开发应用提供了新的途径。
OBJECTIVE: To investigate the effect of high frequency nebulization of multilactic-co-glycolic acid (PLGA) nanoparticles on inflammatory colonic mucosa 5AC (MUC5AC). Methods: Theaflavin PLGA nanoparticles were prepared by modified method. The mean diameter of nanoparticles was detected by particle size analyzer. The entrapment efficiency, drug loading and release in vitro were tested. The SD rats were divided into three groups: control group (group A), cigarette inhalation group (group B) and intervention group (group C). The mRNA expression of mucin 5AC m RNA in each group was detected by RT-PCR. The expression of MUC5AC protein was observed by enzyme-linked immunosorbent assay (ELISA) and histopathological changes were compared. RESULTS: The prepared theaflavins PLGA nanoparticles were stable in nature and their mean particle size was (108.24 ± 2.86) nm, which was suitable for nebulization. Compared with the control group, the expression of MUC5AC protein in cigarette smoke group was significantly increased (P = 0.000), while compared with the cigarette group, the expression of MUC5AC protein was significantly decreased (P = 0.001) after treatment with the theaflavin PLGA nanoparticles. Similar results were obtained with RT-PCR of MUC5AC m RNA expression. Airway histochemistry PAS staining also suggested that inhaled theaflavin PLGA nanoparticles, airway surface mucin expression decreased significantly. CONCLUSION: Theaflavins PLGA nanoparticles prepared by the modified method are stable in nature and uniform in size. Inhalation by nebulization can play a role in the hypersecretion of mucin 5AC that inhibits the inflammatory airway, providing a new method for the development and application of theaflavins way.