论文部分内容阅读
采用L-苯丙氨酸为探针,使用液相色谱仪(HPLC)荧光检测器检测了7种过渡金属离子参与类Fen-ton反应产生.OH的能力.试验采用的激发波长277nm,发射波长306nm.产物L-Tyr在反应前后的荧光变化可间接反映.OH产生量.实验结果表明Cr(Ⅲ)、Fe(Ⅱ)、Co(Ⅱ)、Cu(Ⅱ)、Pb(Ⅱ)产生.OH的能力较强,而Ni(Ⅱ)和Cd(Ⅱ)产生.OH的能力极弱..OH清除剂脱铁敏或甘露醇,能明显抑制产物L-Tyr的荧光强度变化,说明.OH的产生受到抑制,结果表明HPLC方法准确可靠.使用分光光度法对上述离子参与类Fenton反应产生.OH的实验进行了验证,得到了基本一致的结果,但芳环羟基化荧光法的结果更稳定,灵敏度更高,故此方法对于.OH检测以及抗.OH机理和应用研究等方面具有较高应用价值.
Using L-phenylalanine as a probe, the ability of seven transition metal ions to participate in the Fen-ton reaction to produce .OH was investigated by using a liquid chromatographic (HPLC) fluorescence detector. The excitation wavelength used in the experiment was 277 nm and the emission wavelength 306nm.The fluorescence change of L-Tyr before and after reaction can indirectly reflect the amount of OH produced.Experimental results show that Cr (Ⅲ), Fe (Ⅱ), Co (Ⅱ), Cu (Ⅱ) and Pb , While the ability of Ni (Ⅱ) and Cd (Ⅱ) to produce .OH is very weak. The dehydroxybenzamine or mannitol of OH scavenger can significantly inhibit the change of fluorescence intensity of L-Tyr product, The results showed that HPLC method was accurate and reliable.Using spectrophotometry to validate the above-mentioned ion-generated Fenton-like reaction to produce .OH, the results were basically the same, but the result of aromatic ring hydroxylation fluorescence method was more stable, The sensitivity is higher, so this method has high application value for the detection of .OH and the study of mechanism and application of anti-.OH.