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目的分离人知的 c DNAs。方法采用 RT- PCR自人的肝脏细胞分离到一约 1,2 0 0 bp的 c DNA片段 ,将其克隆测序。结果在这一 c DNA片段中包含了两个开放阅读框架。结论一个开放阅读框架代表与人疱疹病毒大衣壳蛋白同源的多肽 ,另一个代表与沙门氏菌外膜蛋白 rck同源的多肽
Objective To isolate human c DNAs. METHODS: An approximately 1,200 bp cDNA fragment of c DNA was isolated from human liver cells by RT-PCR and cloned and sequenced. As a result, two open reading frames are contained in this c-DNA fragment. CONCLUSION: An open reading frame represents a polypeptide homologous to human herpesvirus capsid protein and the other represents a polypeptide homologous to the rck of salmonella outer membrane protein