论文部分内容阅读
目的 探讨胎儿宫内乙型肝炎病毒 (HBVDNA)感染的实验诊断方法及其临床意义。方法 应用酶联免疫吸附试验 (ELISA)和荧光定量聚合酶链反应 (FQ -PCR)对 15 6例乙肝表面抗原 (HBsAg)阳性孕妇的母血和产后婴儿脐血及婴儿外周血的乙型肝炎血清学标志物 (HBV -M)和HBVDNA进行检测。结果 15 6例HDsag阳性孕妇所生婴儿脐血和婴儿外周血HBsAg的检出率为 8.3% (13/ 15 6 )、6 .4 (10 / 15 6 ) ,HBeAg检出率分别为 7.1% (11/ 15 6 )、5 .1% (8/ 15 6 ) ,母血、脐血、婴儿外周血HBVDNA阳性检出率分别为 36 .5 % (5 7/ 15 6 )、2 8.2 % (44 / 15 6 )、2 3.7% (37/ 15 6 ) ;5 7例HBVDNA阳性孕妇血病毒含量 (拷贝数 /ml的对数值 )为 7.34± 2 .2 8,37例阳性婴儿外周血HBVDNA含量为 6 .0 8± 0 .96 ;与婴儿外周血检测结果相比较 ,脐血中HBsag、HBeAg的阳性率不仅存在 2 3.1%和 37.5 %的假阳性 ,HBVDNA也存在 16 .7% (7/ 4 2 )的假阳性 ;在HBeAg或HBVDNA阳性孕妇中 ,婴儿外周血HBVDNA的检出率分别为 73.8% (31/ 4 2 )、6 4 .9% (37/ 5 7) ,显著高于HBeAg或HBVDNA阴性者 5 .3% (6 / 114 ) ,(P <0 .0 1) ;婴儿外周血中HBVDNA阳性率随孕妇HBVDNA含量增加而显著增加(P <0 .0 0 5 ) ,其HBVDNA的含量与母血呈正相关 ,(r =0
Objective To investigate the experimental diagnosis and clinical significance of intrauterine infection of hepatitis B virus (HBVDNA). Methods Serum from 15 6 HBsAg positive pregnant women and postpartum infant cord blood and infant’s peripheral blood were detected by enzyme-linked immunosorbent assay (ELISA) and fluorescence quantitative polymerase chain reaction (FQ-PCR) Serological markers (HBV-M) and HBVDNA were detected. Results The positive rates of HBsAg in cord blood and infants with HBsAg in pregnant women were 8.3% (13/156) and 6.4% (10/156), respectively, and the positive rates of HBeAg in 15 6 HDsag positive pregnant women were 7.1% The positive rates of HBVDNA in maternal blood, umbilical blood and infant were 36.5% (5/7/15) and 8.2% (44%), respectively / 15 6), and 2 3.7% (37/156) respectively. Serum HBV DNA levels in 5 7 HBVDNA positive pregnant women (log / copy number / ml) were 7.34 ± 2.28. HBVDNA content in peripheral blood of 37 positive infants 6 .0 8 ± 0.96; Compared with the results of infant peripheral blood, the positive rates of HBsag and HBeAg in cord blood were not only false positives of 23.1% and 37.5%, but also HBVDNA of 16.7% (7/4 2). In HBeAg or HBVDNA positive pregnant women, the detection rate of HBVDNA in infants was 73.8% (31/42) and 64.9% (37/57) respectively, which was significantly higher than that of HBeAg or The positive rate of HBVDNA in peripheral blood of infants was significantly increased with the increase of HBVDNA in pregnant women (P <0.05), and the positive rate of HBVDNA in HBVDNA was 5.3% (6/114) (P <0.01) The content was positively correlated with maternal blood (r = 0