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本文旨在确定文冠果壳皂苷作为减肥因子对胰脂肪酶活性的作用效果。通过对胰脂肪酶反应条件(pH、反应时间和反应物加入顺序)的摸索,确定AB-8大孔树脂纯化的文冠果壳皂苷对胰脂肪酶活性的影响,并通过紫外吸收光谱(200~340 nm)、荧光光谱(激发波长290 nm,扫描波长300~450 nm)确定文冠果壳皂苷对胰脂肪酶作用的机理。研究结果表明,文冠果壳皂苷对胰脂肪酶抑制率高达87.5%,最佳反应体系pH 7.4,反应时间15 min,反应物加入顺序为底物与文冠果壳皂苷预热后加入胰脂肪酶溶液反应。文冠果壳皂苷与胰脂肪酶作用后,改变了胰脂肪酶中发光氨基酸(Trp、Tyr)所在微环境,胰脂肪酶分子结构和构象发生变化,导致胰脂肪酶紫外吸收光谱最大吸收波长红移和荧光强度降低,胰脂肪酶活性抑制。文冠果壳皂苷可通过抑制胰脂肪酶发挥减肥因子的作用。
The purpose of this paper is to determine the effect of Xanthomonas campestris pachyrhizin as a weight-loss factor on pancreatic lipase activity. The effects of AB-8 macroporous resin purified Panax ginseng saponins on the activity of pancreatic lipase were determined by exploring the reaction conditions of pancrease lipase (pH, reaction time and reactant addition order) ~ 340 nm), and the fluorescence spectrum (excitation wavelength 290 nm, scanning wavelength 300-450 nm) was used to determine the mechanism of action of husk saponin on pancreatic lipase. The results showed that the inhibition rate of Panax ginseng saponins to pancreatic lipase was as high as 87.5%, the optimal reaction pH was 7.4 and the reaction time was 15 min. Enzyme solution reaction. The action of Panax ginseng saponins and pancreatic lipase changed the microenvironment of the light-emitting amino acids (Trp, Tyr) in pancreatic lipase, and the molecular structure and conformation of the pancreatic lipase changed, resulting in the maximum absorption wavelength of pancreatic lipase Migration and fluorescence intensity decreased, pancreatic lipase activity was inhibited. Xanthoceras sorbifolia can exert the effect of weight-loss factor by inhibiting pancreatic lipase.