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为了探讨筛选烟草耐低钾突变体的最优条件,以烟草野生型品种‘中烟100’为材料,研究了不同钾离子浓度对其根生长的影响,确定了在培养基中筛选耐低钾突变体的适宜钾离子浓度为0.075 mmol·L-1。然后以EMS诱变处理获得的‘中烟100’M2代种子为材料,进行了大规模的耐低钾突变体的筛选,初步筛选出了99个M2代候选材料。选取50个材料继续进行了验证,其中22个M3代突变体经复选鉴定弯根率≥75%,这些突变体在低钾培养基上长势明显好于对照,且绝大多数突变体的单位鲜重叶片的钾含量明显高于对照。研究结果可为烟草耐低钾突变体的筛选提供依据。
In order to explore the optimum conditions for screening low potassium tolerant mutants, the effects of different potassium concentrations on root growth were studied using ’Zhongyan 100’, a wild tobacco variety, and the effects of low potassium tolerance The suitable potassium concentration of the mutant was 0.075 mmol·L-1. Then, a large-scale resistance mutant of low potassium was screened from the ’Zhongyan 100’M2 seed obtained by EMS mutagenesis, and 99 M2 candidate materials were screened out. Fifty materials were selected for further validation. Twenty-two of the three M 3 mutants were identified as having a bent-root yield of ≥75%. These mutants grew significantly better on low-K medium than controls, and most of the mutants’ units Fresh weight of the potassium content was significantly higher than the control. The results can provide a basis for the screening of tobacco plants resistant to low potassium.