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本实验采用核桃成熟种胚的上胚轴以上部分为试材，采用不同的表面灭菌处理、基本培养基和生长调节物质浓度，研究各种处理对核桃离体胚生长发育的影响。结果表明：（１）未经表面灭菌处理的处植体生长发育明显优于经表面灭菌处理的外植体；（２）６－ ＢＡ有削弱顶端优势的作用，浓度越高，强度越大；（３）诱导顶芽、侧芽和丛生芽生长的适宜培养基配方和培养条件为：启动培养基１／４ ＭＳ＋ ０．５ ｍ ｇ／Ｌ ＢＡ 或１／２ ＭＳ＋ １．０ ｍ ｇ／ＬＢＡ，待外植体萌发后分别转入１／２ ＭＳ＋ ０．５ ｍ ｇ／Ｌ ＢＡ 或１／２ ＭＳ＋ １．０ ｍ ｇ／Ｌ ＢＡ，待材料进一步发育后分别转入ＭＳ＋ ０．５ ｍ ｇ／ＬＢＡ 或ＭＳ＋ １．０ ｍ ｇ／ＬＢＡ，以上各种培养基均含３０ ｇ／Ｌ蔗糖和７ｇ／Ｌ琼脂，ｐＨ５．８，培养条件为温度２５℃，光照１６ 小时／天，光强约２ ０００ Ｌｕｘ；（４）在无激素１／４ ＭＳ培养基上，具有一定大小的材料可形成不定根，说明低浓度无激素培养基适于核桃离体胚培养物的生根。
In this experiment, the above part of the epicotyl of walnut mature embryos was used as the test material, and the effects of various treatments on the growth and development of walnut in vitro embryos were studied by using different surface sterilizing treatments, basic medium and growth regulators. The results showed that: (1) The growth and development of the implants without surface sterilization were obviously better than that with the surface-sterilized explants; (2) The effect of 6- (3) The suitable medium for inducing the growth of top buds, lateral buds and bud buds was as follows: Startup medium 1/4 MS + 0.5 m g / L BA or 1/2 MS + 1.0 m g / LBA. The explants were transferred to 1/2 MS + 0.5 m g / L BA or 1/2 MS + 1.0 m g / L BA after germination. After further development, the explants were transferred to MS + 0.5 m g / LBA, or MS + 1.0 m g / LBA. The above mediums contained 30 g / L sucrose and 7 g / L agar at pH 5.8. The culture conditions were 25 ° C for 16 hours / About 2 000 Lux; (4) hormone-free 1/4 MS medium, with a certain size of the material can form adventitious roots, indicating that low concentrations of hormone-free culture Roots are suitable for rooting of walnut in vitro embryo cultures.