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为研究10种农药对绿僵菌生长的影响情况,选用10种不同农药,稀释成不同浓度分别加入到SDAY培养基中,用直径为4 mm的无菌打孔器在其中央打孔,然后吸取50μL绿僵菌孢子悬浮液分别注入孔中,置温度25℃、湿度80%的条件下培养,十字法测量菌落的直径和观察孢子颜色变化。结果表明:杀菌剂多菌灵对绿僵菌的生长抑制作用最强,生物化学农药吡虫啉对绿僵菌的生长抑制作用最弱;随着农药浓度的降低,所试农药对绿僵菌的生长抑制作用也随之降低。通过测定10种农药与绿僵菌的相容性,选择生物相容性好的农药以低剂量与之配伍,既可使菌剂增效,又可大幅度降低农药用量,为开发防治害虫的绿僵菌制剂奠定基础。
In order to study the effects of 10 pesticides on the growth of Metarhizium anisopliae, 10 different pesticides were selected and diluted into SDAY medium at different concentrations, punched with a sterile 4 mm diameter hole punch in the center, Pipette 50μL Metarhizium sp. Suspension were injected into the hole, set the temperature 25 ℃, humidity 80% under the conditions of culture, cross measurement of colony diameter and observation spore color change. The results showed that the fungicide carbendazim had the strongest inhibitory effect on the growth of Metarhizium anisopliae and the imidacloprid, the biochemical pesticide, had the weakest inhibitory effect on Metarhizium anisopliae. With the decrease of pesticide concentration, the growth of Metarhizium anisopliae Inhibition also will be reduced. By determining the compatibility of 10 kinds of pesticides with Metarhizium anisopliae, the selection of pesticides with good biocompatibility is compatible with low doses, which not only can enhance the efficiency of the fungicide, but also greatly reduce the dosage of pesticides. In order to develop pest control Metarhizium preparations laid the foundation.