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为了有效地利用固氮菌肥以提高土壤肥力,需要筛选固氮力强、适应性广的高效菌种。现将有关固氮菌的分离、纯化和固氮效能测定方法简介如下: 一、固氮菌的分离法固氮菌的分离通常采用泥盘法、液体分离法。我们目前用的是大盘点土法。分述如下: 1.泥盘法:取不同类型土壤及不同作物根际土壤(一般10厘米左右耕层土)样品50克,加入甘露醇(或葡萄糖)1克及3%磷酸氢二钾5毫升与水调成泥酱,在直径6厘
In order to effectively use nitrogen-fixing bacterium fertilizer to improve soil fertility, we need to screen efficient nitrogen-fixing strains with wide adaptability. Azotobacter now about the separation, purification and determination of nitrogen fixation efficiency are summarized as follows: First, the separation of azotobacter Azotobacter commonly used mud plate separation method, liquid separation method. We are currently using the stock market method. It is divided as follows: 1. Sludge disc method: Take different types of soil and different crops rhizosphere soil (usually about 10 cm layer soil) sample 50 g, add mannitol (or glucose) 1 g and 3% dipotassium hydrogen phosphate 5 Ml and water into a mud sauce, 6% in diameter