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1983年7月,从山东薛城的花生上分离到一个病毒分离物PS—34,以汁液摩擦接种测定了9科48种植物,PS—34可侵染6科15种植物,在苋色藜上表现系统花叶。由桃蚜、豆蚜以非持久性方式传病。体外抗性测定,失毒温度50—55℃,稀释限点10~(-3)—10~(-4),体外存治期3—4天.提纯后经电镜观察,病毒粒体呈球形,直径±29nm.提纯病毒的抗血清和花生矮化病毒日本株系(PSV—J)的抗血清交互测定,都与PS—34有明显的沉淀线反应。故将病毒分离物PS—34鉴定为黄瓜花叶病毒组的花生矮化病毒(PSV)。因苋色藜和昆诺藜上表现为系统花叶,与在寄主反应上明显不同.因此,确定其为一种新的花生矮化病毒PSV—C株系.
In July 1983, a virus isolate PS-34 was isolated from the peanut in Xuecheng, Shandong Province. Fourteen families of 48 species of plants were identified by frictional inoculation. PS-34 was able to infect 6 families and 15 species of plants. On the performance system mosaic. By Myzus persicae, bean aphid disease in a non-persistent manner. In vitro resistance assay, the temperature of detoxication was 50-55 ℃, the dilution limit was 10 -3 -10 -4, and the storage period was 3-4 days in vitro.After purification, the virus particles were spherical , ± 29nm in diameter.The antisera of antisera of purified virus and peanut dwarf virus Japanese strain (PSV-J) were interdependently determined, and all had obvious precipitation reaction with PS-34. The virus isolate PS-34 was identified as the peanut dwarf virus (PSV) of the cucumber mosaic virus group. Because the amaranth and quinoa appeared as systematic mosaic, which was significantly different from the host reaction, it was identified as a new peanut dwarf virus PSV-C strain.