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应用斑点杂交方法对36例HBsAg阴性的各种类型的乙型肝炎患者进行了血清HBV-DNA的检测,33例获得阳性结果。目前认为血清和肝组织HBV-DNA的检测是判断HBV感染和复制的特异和敏感方法,对于HBV感染的临床诊断和流行病学的研究有一定意义。
36 cases of HBsAg-negative hepatitis B patients were tested for HBV-DNA by dot-blot hybridization and 33 cases were positive. At present, it is considered that the detection of HBV-DNA in serum and liver tissue is a specific and sensitive method to judge HBV infection and replication, which is of certain significance for the clinical diagnosis and epidemiological study of HBV infection.