论文部分内容阅读
目的:明确丹皮酚对Hacat细胞增殖及凋亡的影响。方法:培养人角质形成细胞株Hacat,采用WST-8检测细胞丹皮酚对Hacat细胞增殖的影响;流式细胞仪检测丹皮酚对Hacat细胞细胞周期及凋亡的影响。结果:丹皮酚能有效抑制Hacat细胞的增殖,将Hacat细胞阻滞于G2/M期,同时丹皮酚可有促进Hacat细胞凋亡作用。结论:丹皮酚可抑制角质形成细胞的增殖,促进角质形成细胞的凋亡,可能是牡丹皮治疗银屑病的有效单体之一。
Objective: To clarify the effect of paeonol on proliferation and apoptosis of Hacat cells. Methods: The human keratinocyte cell line Hacat was cultured and the effect of paeonol on proliferation of Hacat cells was detected by WST-8. The effect of paeonol on the cell cycle and apoptosis of Hacat cells was detected by flow cytometry. Results: Paeonol could effectively inhibit the proliferation of Hacat cells, arresting Hacat cells in G2 / M phase, and paeonol could promote the apoptosis of Hacat cells. Conclusion: Paeonol can inhibit the proliferation of keratinocytes and promote the apoptosis of keratinocytes, which may be one of the effective monomers for the treatment of psoriasis with tree bark.