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利用小白鼠进行传代是弓形虫传代和保种最常用和最基本的方法。多年来,我所用该法保存了多株弓形虫,进行了数千次传代工作,取得了良好的效果和有益的经验。本文较详细地介绍了4种用小鼠进行弓形虫传代和保种的方法,并对其优缺点进行了讨论。 为了克服常规小鼠传代法传代间隔短、次数频的缺点,我们将上代感染小鼠腹水的生理盐水稀释液放入4℃冻箱保存,存放10～20天后再转种下代。在25次试验中,转种成功率达97.2％。这种改良后的方法较上述常规传代法节省人力、物力,并可根据工作需要较灵活地掌握传代时间。
The passage and passage of mice is the most common and basic method of passage and preservation of Toxoplasma gondii. Over the years, I have used the Act to save more strains of Toxoplasma gondii, carried out thousands of times, and achieved good results and useful experiences. In this paper, four methods of passage and preservation of Toxoplasma gondii in mice are introduced in more detail, and their advantages and disadvantages are discussed. In order to overcome the shortcomings of short passage and frequent frequency in the passage of the conventional mice, we put the normal saline diluted in ascites from the previous generation into the freezer at 4 ℃ and store it for 10-20 days before transplanting the next generation. In 25 trials, the success rate of transplanting was 97.2%. The improved method saves manpower and material resources compared with the conventional method of passage, and can passaging time more flexibly according to the needs of work.