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目的:观察一氧化氮合酶(NOS)抑制剂左旋硝基精氮酸甲酯(L-NAME)长期治疗对四氯化碳所致肝硬化大鼠血流动力学影响。方法:应用~(57)Co 标记微球技术检测肝硬化大鼠血流动力学参数;应用荧光法测定大鼠血清一氧化氮(NO)含量。结果:肝硬化大鼠全部出现高动力循环状态。L-NAME 治疗组高动力循环状态明显改善,血清 NO 含量较未治疗组显著降低(1.47±0.91μmol/L 与4.20±1.25μmol/L,P 值<0.01)。结论:内源性 NO 在肝硬化血流动力学改变中起重要作用。长期小剂量 L-NAME 治疗可明显改善肝硬化大鼠的高动力循环状态。
Objective: To observe the long-term effects of nitric oxide synthase (NOS) inhibitor L-NAME on hemodynamics in cirrhotic rats induced by carbon tetrachloride. Methods: The hemodynamic parameters of cirrhotic rats were detected by ~ (57) Co-labeled microspheres. The level of serum nitric oxide (NO) was measured by fluorescence method. RESULTS: All cirrhotic rats developed hyperdynamic circulation. The state of hyperdynamic circulation in L-NAME treatment group was significantly improved. The serum NO level was significantly lower than that in untreated group (1.47 ± 0.91μmol / L vs 4.20 ± 1.25μmol / L, P <0.01). Conclusion: Endogenous NO plays an important role in the hemodynamic changes of cirrhosis. Long-term low-dose L-NAME treatment can significantly improve the hyperdynamic circulation in cirrhotic rats.