Objective: To investigate theanti apoptosis effect of curcumin (cur) in experimental autoimmune encephalomyelitis (EAE) mice by regulating TLRs/NF-κB signaling pathway and its mechanism. Methods: 45 C57BL/6 mice were randomly divided into the control group,EAE group,curcumin group ,15 mice in each group. Blank groups are not processed. The EAE model was established by classical modeling method in the EAE group and the curcumin group. From the day of modeling, the blank group and the EAE group were intraperitoneally injected with 1ml/kg/d of normal saline, Curcumin group was given 100 mg/kg/d continuous intraperitoneal injection of curcumin extract. With Benson EAE group and Curcumin group mice were killed at the peak of the disease. The blank group and the rest of the mice were killed after 4 weeks of feeding, and the spinal cord tissue was taken out to separate the lumbar enlargement segment. The effects of curcumin on the pathological changes of spinal cord tissue in EAE mice were observed by HE staining and TUNEL staining, and the expression of apoptosis positive cells was calculated. The distribution and co aggregation of apoptosis related proteins Bcl-2 and Bax with spinal cord tissue were observed by double immunofluorescence staining The protein levels of TLR4, NF-κBp65 and MyD88 were detected by Western blot. Results: compared with the blank group, TUNEL staining increased the number of apoptotic cells and the apoptotic rate in EAE group (P<0.05); the expression of apoptosis related protein Bcl-2 decreased and the expression of Bax increased in EAE group (P<0.05) ,The protein of TLR4, NF-κBp65 and MyD88 in spinal cord tissue of mice were increased by blot detection (P<0.05); compared with EAE group, the number of apoptotic cells in spinal cord tissue of curcumin group was decreased by TUNEL staining, and the apoptosis rate was decreased (P<0.05); the expression of apoptosis related protein Bcl-2 was increased, the expression of Bax protein was decreased, and Western blot was used to detect the expression of apoptosis related protein The protein of TLR4, NF-κBp65 and MyD88 in spinal cord tissue of mice were decreased by blot (P<0.05). Conclusion: Curcumin has anti apoptotic effect on EAE mice, and its mechanism may be related to the inhibition of TLRs / NF-κB signaling pathway and the reduction of apoptotic protein production.