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Objective: To explore the inhibition of the expression of c-Myc in human hepatocellular carcinoma via vector-based RNA interference (RNAi).Methods: Two RNA interference DNA templates targeting c-Myc oncogene were designed via online software and synthesized.By ligation,the fragments were inserted into pSilencer 1.0-U6 to construct the recombinant siRNA expressing plasmids.The identified recombinants were introduced into BEL-7402 cells with Lipofactamine.The inhibition of c-Myc expression,together with the expression of CDK4,hTERT and Gadd45β in c-Myc down-regulated BEL-7402 cells,were analyzed by semi-quantitative RT-PCR.Results: Two recombinant plasmids pSic-myc-1 and pSic-myc-2,which direct the yields of siRNAs targeting c-Myc in cells,were constructed.Among which,pSic-myc-2 was shown to trigger a RNAi-mediated inhibition of expression of c-Myc in BEL-7402 by up to 90%.In c-Myc knockdown BEL-7402 cells,the expression of CDK4 and hTERT were down-regulated with a ratio of 85% and 57%,respectively,while the expression of Gadd45β was up-regulated by up to 110%.Conclusion: The expression of c-Myc in BEL-7402 could be suppressed by vector-based RNA interference successfully.The knockdown of c-Myc in tu resulted in the changes of expression of genes related to cell proliferation and apoptosis.Thus,our study provided a preliminary data in searching of a c-Myc-targeted RNAi therapy of human hepatocellular carcinoma.