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为了解天津团泊湖地区盐碱土中可培养耐盐碱细菌的多样性,以HorikoshiⅡ为基础培养基,在pH 7~9.5、NaCl浓度0~15%范围内,对天津团泊湖地区盐碱土土壤样品中可培养耐盐碱细菌进行分离,并对它们进行16S rDNA序列测定和系统进化分析,共得到不同表型的细菌75株。结果表明,这些分离菌株分别属于芽孢杆菌属(Bacillus)、微小杆菌属(Exiguobacterium)、海洋芽孢杆菌属(Oceanobacillus)、鱼露芽孢杆菌属(Jeotgalibacillus)和微球菌属(Micrococcus)。芽孢杆菌属和海洋芽孢杆菌属是天津团泊湖可培养耐盐碱菌的优势细菌种群,它们分别占已测种群的48%(36株)和24%(18株)。大多数菌株与已知的系统发育关系密切的已知典型菌株之间存在一定的遗传差异(16S rDNA序列的相似性为99.6%~99.9%),其中至少有4个菌株(TJTB06、TJTB32、TJTB50和TJTB51)代表潜在的新菌株。天津团泊湖地区存在较为丰富的耐盐碱菌种群,并且潜藏着较多的新种种源,极具进一步发掘研究的价值。
In order to understand the diversity of saline-alkali-tolerant bacteria cultivable in saline-alkali soil in Tuanpo Lake in Tianjin, HorikoshiⅡwas used as the basal medium. The pH value of saline soil Soil samples can be cultured salinity-resistant bacteria were isolated, and their 16S rDNA sequence analysis and phylogenetic analysis, a total of 75 strains of different phenotypes were obtained. The results showed that these isolates belong to the genus Bacillus, Exiguobacterium, Oceanobacillus, Jeotgalibacillus and Micrococcus, respectively. Bacillus and marine Bacillus are the dominant bacterial populations that can cultivate Saline-resistant bacteria in Tuanbo Lake, Tianjin, accounting for 48% (36 strains) and 24% (18 strains) of the tested populations, respectively. Most of the strains have some genetic differences with the known typical strains (16.6% ~ 99.9% of the 16S rDNA sequences), of which at least 4 strains (TJTB06, TJTB32, TJTB50 And TJTB51) represent potential new strains. There is abundant salt-tolerant algae population in Tuanbo Lake, Tianjin, and there are many new provenances, which greatly explore the value of the research.