研究原癌基因pim-2的分子生物学特性,分析其相关机制。使用生物信息学方法和技术研究分析原癌基因pim-2,用在线服务器分析pim-2基因的染色体定位,预测外显子、开放式阅读框、CpG岛和miRNAs互补片段等。用生物信息学软件预测原癌基因pim-2转录蛋白的理化性质和蛋白序列各类修饰位点,如泛素化、糖基化等,计算抗原指数,模建空间结构。结果显示原癌基因pim-2有6个外显子,CDS编码框转录一段含311个氨基酸的肽链;基因启动子存在CpG岛;3′UTR区域含miRNA基因。Pim-2蛋白分子量34 188.47,等电点5.78,不稳定指数是45.87,消光系数为279nm;蛋白序列分布着多处共价修饰位点、2处泛素化位点、4处糖基化位点、1处SUMO化位点、1处亚硝基化位点以及2处棕榈酰化位点;蛋白序列存在16段抗原指数较高区域。研究表明原癌基因pim-2序列上所分布的相关区域和修饰位点与其致癌作用存在密切关系。 To study the molecular biology of proto-oncogene pim-2 and to analyze its mechanism. Bioinformatics methods and techniques were used to analyze and analyze proto-oncogene pim-2. The on-line server was used to analyze the chromosomal location of pim-2 gene and predict exons, open reading frames, CpG islands and miRNAs complementary fragments. The bioinformatics software was used to predict the physical and chemical properties of protooncogene pim-2 transcripts and various modification sites of protein sequences, such as ubiquitination and glycosylation. The antigenic index was calculated and the spatial structure was modeled. The results showed that there are 6 exons in the oncogene pim-2. The CDS coding sequence transcribes a 311 amino acid peptide chain; CpG island exists in the promoter of the gene; miRNA gene is located in the 3 ’UTR region. The molecular weight of Pim-2 protein was 34 188.47, the isoelectric point was 5.78, the instability index was 45.87, and the extinction coefficient was 279nm. The protein sequence distributed multiple covalent modification sites, two ubiquitination sites, four glycosylation sites Point, one SUMO site, one nitrosylation site and two palmitoylation sites. The protein sequence has a 16-region higher antigen index. Studies have shown that proto-oncogene pim-2 sequence distribution of the relevant regions and sites of modification and its role in carcinogenesis there is a close relationship.