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基于多重PCR(Polymerase chain reaction)与毛细管五色荧光检测系统,利用36对核心引物构建了138份棉花主栽品种DNA指纹数据库,采用棉花DNA指纹数据库软件管理系统进行数据统计与分析。36对引物在138份材料中共扩增出143个多态性等位位点,每对引物的等位位点数为2～8个,平均每对引物扩增出3.97个多态性等位位点。抽查的101个主栽品种仅45.6%的位点完全纯合,中棉所系列品种61.6%的位点完全纯合。存在5种非纯合位点表现形式,以2种基因型混杂所占比例最大,其中以5∶1的混杂类型最多。4种类型的同名品种指纹比对分析表明,品种内的遗传变异度均在0～2个差异位点。初步建立了高通量的棉花DNA指纹检测与数据分析平台,提出了不同品种间的差异判别标准。
Based on the multiplex PCR (Polymerase chain reaction) and capillary five-color fluorescence detection system, DNA fingerprinting database of 138 cultivars of cotton was constructed by using 36 pairs of core primers. The data was analyzed and analyzed by cotton DNA fingerprinting database software management system. A total of 143 polymorphic alleles were amplified by 36 pairs of primers from 138 accessions. The number of alleles per primer pair ranged from 2 to 8, with an average of 3.97 polymorphic alleles per primer pair point. Only 45.6% of the 101 cultivars tested were completely homozygous, and 61.6% of the cotton cultivars in China were completely homozygous. There are five kinds of non-homozygous loci expression, with the two genotypes mixed the largest proportion, of which 5: 1 mixed type most. Fingerprinting analysis of the four types of the same name species showed that within the variety of genetic variation were in 0 to 2 different sites. A high-throughput cotton DNA fingerprint detection and data analysis platform was initially established, and the criteria for discriminating the differences among different varieties were proposed.