论文部分内容阅读
目的应用real-time RT-PCR和病毒序列测定等方法对福建省首例人感染H7N9禽流感病例开展实验室诊断。方法采集人感染H7N9禽流感病例呼吸道标本并提取RNA,分别采用甲乙型流感通用引物和探针、季节性流感(包括H3N2、H1N1、H1N1pdm)特异性引物和探针以及H7N9特异性引物和探针进行real-time RT-PCR检测。利用自行设计的引物扩增病毒基因组节段,测定并分析病毒基因序列。结果 real-time RT-PCR结果表明,应用甲型通用引物扩增结果阳性,乙型及季节性流感(包括H3N2、H1N1以及H1N1pdm)扩增结果均为阴性,特异性H7N9亚型流感病毒扩增结果阳性。序列测定获得的病毒4个节段的序列与已公布的人感染H7N9禽流感病毒序列高度一致。结论病例呼吸道标本中存在人感染H7N9禽流感病毒,病毒的基因与近期国内流行的人感染H7N9禽流感病毒高度类似。
Objective To carry out laboratory diagnosis of H7N9 bird flu in Fujian province by real-time RT-PCR and virus sequence analysis. Methods Human respiratory tract samples of H7N9 bird flu were collected and RNA was extracted. The primers and probes of influenza A and B, the specific primers and probes of seasonal influenza (including H3N2, H1N1 and H1N1pdm) and H7N9 specific primers and probes Real-time RT-PCR was performed. The virus genome segments were amplified by self-designed primers, and the viral gene sequences were determined and analyzed. Results The results of real-time RT-PCR showed that the amplification results of positive primers A and B were all negative and that of influenza B and seasonal influenza A (including H3N2, H1N1 and H1N1pdm) were negative. The amplification of specific H7N9 influenza virus The result is positive. Sequence analysis of the virus obtained by four segments of the sequence and published human H7N9 highly consistent with the sequence of the bird flu virus. Conclusion Human H7N9 avian influenza virus (H7N9) is present in the respiratory samples. The gene of the virus is highly similar to the H7N9 avian influenza virus recently infected in China.