目的:探讨牛脾肽对荷肝癌小鼠肿瘤生长的抑制作用及其可能机制。方法:建立小鼠H22肝癌细胞实体瘤模型,随机分为3组,分别给5-Fu(20mg/Kg),牛脾肽(0.4mL/只),生理盐水(0.4mL/只),尾静脉注射,隔日给药连续10d。10d后处死小鼠,计算重量抑瘤率;取瘤组织制备石蜡切片,原位杂交法检测瘤细胞VEGFmRNA表达情况并计算瘤组织微血管密度,免疫组化法检测瘤组织中K i67的表达情况,流式细胞仪检测小鼠免疫功能、细胞周期及凋亡。结果:牛脾肽具有较好地抑制肝癌细胞增殖生长及血管生成作用,抑制率为50.49%。且对VEGF mRNA及K i67表达均有较显著的抑制作用(P<0.01),流式细胞仪检测,牛脾肽可以阻滞肿瘤细胞停滞于S期,提高小鼠免疫功能,促进瘤细胞凋亡。结论:牛脾肽具有抑制肝癌细胞生长增殖的作用,其抗肝癌细胞增殖作用机制为诱导肿瘤细胞凋亡,调控细胞周期,增强免疫功能。 Objective: To investigate the inhibitory effect of bovine spleen peptide on tumor growth in hepatoma-bearing mice and its possible mechanism. Methods: The mouse H22 hepatocarcinoma cell solid tumor model was established and randomly divided into 3 groups: 5-Fu (20mg / Kg), spleen peptide (0.4mL / only), normal saline (0.4mL / Injection, the next day administration for 10d. The mice were killed after 10 days to calculate the weight inhibition rate. Paraffin sections were taken from the tumor tissue, the expression of VEGF mRNA was detected by in situ hybridization and the microvessel density was calculated. Immunohistochemistry was used to detect the expression of K i67 in the tumor tissue. Flow cytometry was used to detect immune function, cell cycle and apoptosis in mice. Results: Bovine spleen peptide can inhibit the proliferation and angiogenesis of hepatocellular carcinoma cells with the inhibitory rate of 50.49%. (P <0.01). Flow cytometry showed that bovine spleen peptide could block the tumor cells from arresting in S phase, improve the immune function of mice and promote the apoptosis of tumor cells Death. CONCLUSION: Bovine spleen peptide can inhibit the growth and proliferation of hepatocellular carcinoma cells. Its mechanism of anti-hepatoma cell proliferation is to induce tumor cell apoptosis, regulate cell cycle and enhance immune function.