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目的:观察骨髓增生异常综合征(MDS)患者来源的间充质干细胞(MSC)对混合淋巴细胞反应体系中的T淋巴细胞亚群的影响。方法:将MDS来源的MSC,按照不同比例加入双向混合淋巴细胞培养体系中共同孵育,3d后采用流式细胞术(FCM)和ELISA法检测其表面抗原以及上清液中细胞因子的变化。结果:MDS来源的MSC能明显抑制共孵育体系中的CD8+T细胞亚群和IFN-γ的分泌,轻度抑制IL-4的分泌,与正常来源的MSC抑制性没有统计学意义。结论:MSC在体外能明显抑制CD8+T细胞(CTL)和Th1细胞,轻度抑制Th2细胞。
Objective: To observe the effect of mesenchymal stem cells (MSCs) derived from patients with myelodysplastic syndrome (MDS) on T lymphocyte subsets in mixed lymphocyte reaction system. METHODS: MSCs derived from MDS were added into mixed culture system of mixed lymphocytes at different ratios for co-incubation. After 3 days, the surface antigens of MSCs and the cytokines in supernatants were detected by flow cytometry (FCM) and ELISA. Results: MSCs derived from MDS could significantly inhibit the secretion of CD8 + T cell subsets and IFN-γ in co-incubation system and mildly inhibit the secretion of IL-4, which was not statistically different from that of normal MSC. Conclusion: MSC could significantly inhibit CD8 + T cells (CTL) and Th1 cells in vitro and slightly inhibit Th2 cells.