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目的:观察加味生脉散对大鼠缺血再灌注肾脏损伤的保护作用。方法:双侧肾动脉夹闭45min,再灌注24h,造成大鼠缺血再灌注肾脏损伤模型。分别测定加味生脉散治疗组、病理模型组、假手术组大鼠血尿素氮(BUN)、血肌酐(Cr)、组织中髓过氧物酶(MPO)和丙二醛(MDA)浓度,并进行组织病理学检查。结果:与病理模型组相比,加味生脉散治疗组的缺血再灌注大鼠血尿素氮下降42.8%(P=0.024),血清肌酐下降55.9%(P=0.011);肾脏组织MPO及MDA含量明显降低(P<0.01);组织病理学改变明显减轻(P<0.05)。结论:加味生脉散可减轻缺血再灌注引起的肾脏损伤,其机制与降低缺血再灌注有关的自由基损伤和炎性损伤有关。
Objective: To observe the protective effect of Modified Shengmai Powder on renal injury induced by ischemia-reperfusion in rats. METHODS: The bilateral renal arteries were clamped for 45 minutes and then reperfusioned for 24 hours, which caused a rat model of ischemia-reperfusion kidney injury. The concentrations of blood urea nitrogen (BUN), serum creatinine (Cr), myeloperoxidase (MPO) and malondialdehyde (MDA) in the treated group were measured, the pathological model group and the sham operation group were added. And histopathological examination. RESULTS: Compared with the pathological model group, blood urea nitrogen was decreased by 42.8% (P=0.024), serum creatinine was decreased by 55.9% (P=0.011), and MPO and MDA were observed in kidney tissue of the rats treated with Shengmai Powder. The content was significantly decreased (P<0.01); histopathological changes were significantly reduced (P<0.05). Conclusion: Modified Shengmaisan can reduce the renal damage caused by ischemia-reperfusion, and its mechanism is related to the reduction of free radical damage and inflammatory injury associated with ischemia-reperfusion.