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目的:探讨瘦素及其受体的表达与大肠癌临床病理之间的关系及与大肠癌发生、发展及浸润转移的相关性。方法:应用RT-PCR、免疫组化方法检测80例大肠癌、35例大肠腺瘤标本中瘦素、瘦素受体mRNA及蛋白的表达,分别比较不同临床分期、组织分化、年龄和性别中二者的表达情况。结果:瘦素、瘦素受体mRNA及蛋白在大肠癌组织和大肠腺瘤组织中均有表达,在大肠癌组织中的表达水平明显高于大肠腺瘤组(P<0.05)。但在大肠癌组织分化、患者的年龄和性别之间差异无统计学意义(P>0.05)。结论:大肠癌组织及大肠腺瘤组织中均存在瘦素、瘦素受体mRNA及蛋白,癌组织中有更高的检出率,临床晚期较临床早期的患者有更高的表达,其表达同组织分化程度、年龄和性别无关。瘦素及其受体的异常表达与大肠癌的发生、发展有一定的关系。
Objective: To investigate the relationship between the expression of leptin and its receptor and the clinicopathology of colorectal cancer and its correlation with the occurrence, development, invasion and metastasis of colorectal cancer. Methods: The expressions of leptin and leptin receptor mRNA and protein in 80 cases of colorectal carcinoma and 35 cases of colorectal adenoma were detected by RT-PCR and immunohistochemistry. The differences of clinical stage, The expression of both. Results: The expressions of leptin and leptin receptor mRNA and protein in colorectal carcinoma and colorectal adenoma were significantly higher than those in colorectal adenoma (P <0.05). However, in the differentiation of colorectal cancer tissues, there was no significant difference in the age and sex of the patients (P> 0.05). CONCLUSION: Both leptin and leptin receptor mRNA and protein are present in colorectal carcinoma and colorectal adenoma tissues. The detection rate of leptin and protein in cancer tissues is higher than that in early clinical stage. The expression of leptin, Differentiation with the organization, age and gender have nothing to do. The abnormal expression of leptin and its receptor has a certain relationship with the occurrence and development of colorectal cancer.