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研究新型干扰素hIFN-λ1和hIFN-ε基因在WI-38细胞中的表达及其生物学活性,并进行对比分析。构建His重组融合表达载体pcDNA3.1A-hIFN-λ1-His和pcDNA3.1A-hIFN-ε-His,脂质体法分别转染WI-38人胚肺细胞。采用微量细胞病变抑制试验研究和比较rhIFN-λ1-His和rhIFN-ε-His的抗病毒活性;MTT法检测其对肿瘤细胞生长的影响; RT-PCR相对定量法检测其诱导WI-38和SHG-44细胞产生人MxA抗病毒蛋白的活性。结果表明rhIFN-λ1-His抗病毒活性、抗肿瘤细胞增殖活性和诱导产生MxA蛋白活性要强于rhIFN-ε-His。hIFN-λ1和hIFN-ε干扰素的抗病毒效应的分子机理均与诱导细胞产生MxA抗病毒蛋白相关。为进一步探讨和比较hIFN-λ1和hIFN-ε的生物学活性及其作用机制奠定了一定的基础。
The expression and biological activity of novel interferon hIFN-λ1 and hIFN-ε genes in WI-38 cells were studied and compared. His recombinant fusion expression vector pcDNA3.1A-hIFN-λ1-His and pcDNA3.1A-hIFN-ε-His were constructed and transfected into WI-38 human embryonic lung cells by lipofectamine 2000 respectively. The anti-viral activity of rhIFN-λ1-His and rhIFN-ε-His was studied and compared with the results of MTT assays. The effects of rhIFN-λ1-His and rhIFN-ε-His on the growth of tumor cells were detected by MTT assay. -44 cells produce human MxA antiviral protein activity. The results showed that rhIFN-λ1-His antiviral activity, anti-tumor cell proliferation activity and induction of MxA protein activity than rhIFN-ε-His. The molecular mechanisms of the antiviral effects of hIFN-λ1 and hIFN-ε interferon are all associated with the induction of MxA antiviral proteins by cells. This study laid the foundation for further exploration and comparison of the biological activities of hIFN-λ1 and hIFN-ε and their mechanism of action.