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目的观察人肺腺癌细胞和肺纤维母细胞照射后死亡的特征。方法HE染色观察照射后死亡细胞的形态学改变,Comet检测凋亡细胞内DNA双链断裂,流式细胞仪检测照射后细胞周期的改变及凋亡发生时机。结果肺腺癌细胞照射后出现凋亡,凋亡发生于细胞渡过照射诱导的C2停滞后的G1停滞期;肺纤维母细胞照射后仅见到坏死改变。结论凋亡是肺腺癌细胞照射后增殖死亡的表现形式之一,增殖凋亡的发生具有剂量和时间依赖关系。而增殖坏死可能是肺纤维母细胞照射死亡的主要形式,揭示了不同种类细胞增殖死亡的复杂性和多样性。
Objective To observe the characteristics of death after irradiation of human lung adenocarcinoma cells and lung fibroblasts. Methods HE staining was used to observe the morphological changes of dead cells after irradiation. Comet was used to detect the DNA double-strand breaks in apoptotic cells. Flow cytometry was used to detect the change of cell cycle and the timing of apoptosis. Results Apoptosis occurred after irradiation of the lung adenocarcinoma cells. Apoptosis occurred when cells crossed the irradiation-induced G2 arrest phase of C2 arrest; only necrotic changes were seen after lung fibroblast irradiation. Conclusion Apoptosis is one of the manifestations of proliferation and death after irradiation of lung adenocarcinoma cells. Proliferation and apoptosis occur in a dose- and time-dependent manner. Proliferation and necrosis may be the main form of lung fibroblast death and reveal the complexity and diversity of different types of cell death.