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本实验探讨了小鼠ＣＤ３ＡＫ介导的ＭＨＣ非限制性杀瘤作用途径。ＣＤ３ＡＫ可在体外杀伤小鼠肝癌细胞ＨＣａ－Ｆ和成纤维瘤细胞Ｌ９２９，但其培养上清无杀瘤活性，多聚甲醛固定能明显降低ＣＤ３ＡＫ对Ｈｃａ－Ｆ的杀瘤活性，但对Ｌ９２９的杀瘤活性影响不大，结果表明ＣＤ３ＡＫ的杀瘤机制主要是直接杀伤，膜ＴＮＦ参与ＴＮＦ敏感肿瘤细胞的杀伤
This experiment explored the murine CD3AK mediated MHC non-limiting tumoricidal pathways. CD3AK can kill HCa-F and fibroblast L929 cells in vitro, but its culture supernatant has no cytotoxicity. Paraformaldehyde fixation can significantly reduce the cytotoxic activity of CD3AK against Hca-F, The effect of killing tumor activity was not significant. The results showed that the killing mechanism of CD3AK was mainly direct killing, and the membrane TNF involved in the killing of TNF-sensitive tumor cells