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从４个月胎肝中制备提取物，采用３ＨＴｄＲ掺入、ＭＴＴ比色法和细胞形态学等技术观察了胎肝提取物（ＥＬＥ）对ＢＥＬ７４０２肝癌细胞的影响。１％和２％ＤＭＳＯ对细胞ＤＮＡ合成的抑制率为５６％和５６％；对线粒体脱氢酶的抑制率为４３％和４８％；形态学改变主要表现为有丝分裂相减少，胞质伸出粗细不等的突起，相互连成网状结构。５０５００ｍｇ／Ｌ的ＥＨＥ可明显抑制７４０２细胞的ＤＮＡ合成，并呈剂量作用效应，ＩＣ５０为２４０ｍｇ／Ｌ；对线粒体脱氢酶活力的影响，５００ｍｇ／Ｌ时有轻微的抑制，而５０ｍｇ／Ｌ则有一定的促进作用；对７４０２细胞形态改变与ＤＭＳＯ作用后分化的形态改变相似。以上结果提示，ＥＬＥ可抑制７４０２细胞的增殖并促进其分化。
Extracts were prepared from 4-month fetal liver and 3HTdR incorporation, MTT colorimetry, and cell morphology were used to observe the effect of fetal liver extract (ELE) on BEL7402 hepatoma cells. The inhibition of DNA synthesis by 1% and 2% DMSO was 56% and 56%; the inhibition rate of mitochondrial dehydrogenase was 43% and 48%; the morphological changes were mainly mitotic phase reduction, cytoplasmic stretched thickness Unequal protrusions connect each other into a network structure. 50500mg/L EHE could significantly inhibit DNA synthesis of 7402 cells with a dose-effect effect, IC50 of 240mg/L; effect on mitochondrial dehydrogenase activity was slightly inhibited at 500mg/L, while 50mg/L had A certain promotion effect; the 7402 cell morphology changes similar to the morphological changes after DMSO exposure. These results suggest that ELE inhibits the proliferation of 7402 cells and promotes their differentiation.