松毛虫核型多角体病毒的粗提,根据国内有关资料报道,一般采用离心沉淀方法;多角体的染色过去多采用什维佐娃和有贺久雄法。本试验对多角体病毒粗提物,设计4种提取方法,进行多次比较;同时对多角体病毒的染色方法进行摸索,着重探讨了溴酚兰染液对核型多角体病毒染色的可靠性,现将结果报告如下: 一、提取方法的比较从表1看出,镜检情况及显微摄影都可以 According to the domestic data reported, the general use of centrifugal precipitation method; polyhedron staining in the past to use more Wei Zuo Zova and have a long time male method. In this experiment, four kinds of extraction methods of polyhedrosis virus crude extract were designed and compared for many times. At the same time, the method of staining polyhedrosis virus was explored, and the reliability of bromophenol blue dye staining for nuclear polyhedrosis virus , The results are reported as follows: First, comparison of extraction methods As can be seen from Table 1, microscopic examination and microscopy can be