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目的评价PorA、PorB和Class 4对流感裂解疫苗的免疫增强作用,从中挑选出最有效的流感黏膜佐剂,为发展流感黏膜疫苗提供理论基础。方法流感三价裂解抗原按比例与PorA、PorB和Class4非共价结合,滴鼻免疫Balb/c小鼠3次,采取间接ELISA检测血清特异性IgG抗体及抗体亚型,检测鼻咽、肺、小肠和阴道冲洗液中IgA效价,采用血凝抑制试验检测血清中HAI效价。结果 PorB重组蛋白佐剂组较无佐剂的流感裂解抗原组在提高小鼠早期免疫应答的同时诱导较强的系统免疫应答和黏膜免疫应答;PorA组也有黏膜佐剂的功能,但和无佐剂的流感裂解抗原组相比,差异无统计学意义。结论在蛋白体的三分子中,以PorB为佐剂的流感黏膜疫苗不仅提高了抗原的系统免疫应答,而且诱导了较强的小鼠呼吸道、生殖道的局部黏膜免疫应答,为流感黏膜疫苗的研制奠定了理论基础。
Objective To evaluate the immune enhancement effect of PorA, PorB and Class 4 against influenza flu vaccine and choose the most effective influenza mucosal adjuvant to provide the theoretical basis for the development of influenza mucosal vaccine. Methods Balb / c mice were immunized intranasally three times with PorA, PorB and Class4 non-covalently. Serum specific IgG antibodies and antibody subtypes were detected by indirect ELISA. The nasopharynx, lung, Intestinal and vaginal wash IgA titers, the use of hemagglutination inhibition test serum HAI titers. Results The PorB recombinant protein adjuvant induced a stronger systemic immune response and mucosal immune response than the unadjuvanted influenza lysed antigen group while enhancing the early immune response in mice. PorA also had the function of mucosal adjuvant, The difference was not statistically significant compared with the flu-lysed antigen group. Conclusions PorB-adjuvanted influenza mucosal vaccine not only enhances the systemic immune response of the antigen, but also induces the local mucosal immune response in the respiratory tract and genital tract of mice with high sensitivity for influenza mucosal vaccine Research and laid a theoretical foundation.