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应用过氧化酶标记的链霉卵白素染色疗法，研究转移抑制基因nm23－H1的表达产物二磷酸核奇激酶（NDPK）在人鼻咽癌中的发达及其意义。结果表明，nm23－H1／NDPK表达与鼻咽癌患者的性别、年龄、临床分期无关。在3例正常鼻咽组织中阳性率为100％；在作癌鼻咽粘膜组织上皮细胞中阳性率为100％（7／7）；癌旁组织上皮细胞中阳性率为73．8％（28／38）；鼻咽癌组织细胞中，阳性率为44．7％（17／38），差异有显著意义（P＜0．05）。另外，nm23－H1／NDPK在鼻咽癌无颈淋巴结转移的病例标本（cN0）中阳性率为75．0％（9／12）；在有颈淋巴结转移的病例标本（cN1～3）中阳性率为30.8％（8／26），两者有显著差异（P＜0．05）。提示，nm23－H1／NDPK表达与鼻咽癌患者的淋巴结转移与否是显著负相关，可能在鼻咽癌的淋巴结转移过程中起负性调控作用。
Peroxidase-labeled streptavidin staining was used to study the development of NDPK, a metastasis suppressor gene nm23-H1, and its significance in human nasopharyngeal carcinoma. The results showed that, nm23-H1 / NDPK expression and nasopharyngeal carcinoma patients gender, age, clinical stage has nothing to do. The positive rate was 100% in 3 normal nasopharyngeal tissues and 100% (7/7) in epithelial cells of NPC. The positive rate of epithelial cells in paracancerous tissues was 73.8% (28 / 38). The positive rate of nasopharyngeal carcinoma cells was 44.7% (17/38), the difference was significant (P <0.05). In addition, the positive rate of nm23-H1 / NDPK in nasopharyngeal carcinoma without cervical lymph node metastasis (cN0) was 75.0% (9/12), positive in cN1-3 with cervical lymph node metastasis The rate was 30.8% (8/26), with significant difference (P <0.05). These results suggest that there is a significant negative correlation between the expression of nm23-H1 / NDPK and lymph node metastasis in patients with nasopharyngeal carcinoma, which may play a negative regulatory role in the lymph node metastasis of nasopharyngeal carcinoma.