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为了探讨三七皂苷 (PNS)对人骨髓CD34+ 造血干 /祖细胞的刺激增殖和诱导分化的作用 ,用DynalM 4 5 0CD34+ 免疫磁珠阳性选择法获取高纯度的人骨髓CD34+ 细胞 ,采用多向祖细胞 (CFU Mix)体外集落培养和流式细胞术检测细胞增殖与分化。结果显示 :经免疫磁珠阳性选择 ,从骨髓细胞收获的CD34+ 细胞获得率为 (1.0 3±0 .74 ) % ,流式细胞术分析纯度达到 86 % - 93%。PNS 10mg/L和 2 5mg/L能刺激CD34+ 细胞增殖 ,使CFU Mix集落生成增加 ,2 5mg/L的PNS对CFU Mix产率提高 (34.7± 16 .0 ) % (P <0 .0 1) ,是促进造血的最适浓度 ;PNS2 5 ,5 0和 10 0mg/L时 ,能诱导CD34+ 细胞向粒系细胞分化 ,粒系表面标记CD33+ 和CD15 + 的细胞百分比均明显高于无PNS的对照组 ,而红系细胞表面标记CD71+ 和G A+ 细胞百分比则无明显变化。结论 :PNS对CD34+ 造血干 /祖细胞不但具有显著的刺激增殖作用 ,而且能够诱导其向粒系细胞定向分化的效应
To investigate the effect of notoginsenoside (PNS) on the proliferation and differentiation of human bone marrow CD34+ hematopoietic stem/progenitor cells, a high purity human bone marrow CD34+ cell line was obtained with DynalM 4 5 0CD34+ immunomagnetic beads positive selection method. Cells (CFU Mix) were cultured in vitro and flow cytometry was used to detect cell proliferation and differentiation. The results showed that the yield of CD34+ cells harvested from bone marrow cells was (1.03±0.74)% after immunomagnetic bead positive selection, and the purity of flow cytometry analysis reached 86% - 93%. PNS 10 mg/L and 25 mg/L stimulated the proliferation of CD34+ cells, increased the production of CFU Mix colonies, and increased the yield of CFU Mix by 25 mg/L PNS (34.7±16.0%) (P < 0.01). , It is the optimal concentration to promote hematopoiesis; PNS2 5, 50 and 100 mg/L can induce CD34+ cells to differentiate into granulocyte cells, and the percentage of cells with surface markers of CD33+ and CD15+ is significantly higher than that without PNS. The group, but the percentage of erythroid cell surface markers CD71+ and GA+ cells did not change significantly. Conclusion :PNS can not only stimulate the proliferation of CD34+ hematopoietic stem/progenitor cells, but also induce the directional differentiation of CD34+ hematopoietic cells.