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目的:研究五味子宁神口服液对3T3细胞紫外辐射损伤的保护作用。方法:建立3T3细胞紫外辐射模型,采用流式细胞术检测高、中、低剂量组(12.5,25.0,50.0 mg·ml-1)五味子宁神口服液作用前后细胞内活性氧浓度的变化,检测胞质匀浆中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性以及丙二醛(MDA)含量变化。结果:与中波紫外线辐射组(UVB组)比较,高、中、低剂量组五味子宁神口服液的活性氧含量和MDA含量显著降低(P<0.01),SOD、CAT和GSH-Px活性显著升高(P<0.01或0.05)。结论:五味子宁神口服液能提高SOD、CAT、GSH-Px等酶活性,降低活性氧和MDA含量,对UVB辐射造成的细胞损伤具有保护作用。
Objective: To study the protective effect of Schisandra Ninghuo oral liquid on 3T3 cells exposed to ultraviolet radiation. Methods: The 3T3 cells UV radiation model was established. The changes of intracellular reactive oxygen species in the high, medium and low dose groups (12.5, 25.0, 50.0 mg · ml-1) before and after treatment were measured by flow cytometry. The changes of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activity and malondialdehyde (MDA) Results: Compared with UVB group, the activity of SOD, CAT and GSH-Px significantly decreased (P <0.01) in the high, medium and low dose groups compared with the UVB group (P <0.01 or 0.05). Conclusion: Schisandra Ninghuo Oral Liquid can increase the activities of SOD, CAT, GSH-Px and other enzymes, reduce the reactive oxygen species and MDA content, and protect cells from damage caused by UVB radiation.