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目的改进正常子宫内膜和子宫内膜异位症在位和异位内膜间质细胞原代和传代培养的方法 ,以使其可作为的体外实验模型之一。方法在原代分离细胞、传代培养条件和细胞纯化等方面对子宫内膜间质细胞原代培养的方法作了改进。结果 43例标本培养成功37例,仅1例污染,角蛋白、波形蛋白染色证实细胞纯度达95%以上符合实验要求。结论改进后的子宫内膜间质细胞原代培养法克服了污染问题,增加了可获得的细胞数,子宫内膜间质细胞的分离、培养可作为子宫内膜异位症的体外实验模型之一。
Objective To improve the method of primary and subculture of eutopic and ectopic endometrial stromal cells in normal endometrium and endometriosis so as to make it an experimental model in vitro. Methods The method of primary culture of endometrial stromal cells was improved in the primary separation of cells, subculturing conditions and cell purification. Results 43 cases were successfully cultured in 37 cases, only 1 case of pollution, keratin, vimentin staining confirmed that the purity of cells reached 95% or more in line with experimental requirements. Conclusion The improved primary culture of endometrial stromal cells overcomes the problem of pollution, increases the number of available cells and the separation of endometrial stromal cells, and can be used as an in vitro experimental model of endometriosis one.