本文报道了4℃冰箱保存含弓形体的小鼠生理盐水腹腔洗涤液的保种方法,最长保存时间49天,仍具有感染力和再感染力,有效保存时间为35天。因此,一个月左右,通过一次动物转种,抽取小鼠生理盐水腹腔洗涤液,置4℃冰箱,如此重复,可以达到长期保种目的。此法与其它保种方法相比较,具有简便、易行等优点,值得试用。 This paper reports the method of preserving Toxoplasma gondii peritoneal washes in mice maintained at 4 ℃. The longest preservation time of 49 days is still infectivity and re-infectivity, and the effective storage time is 35 days. Therefore, a month or so, through an animal transplants, mice saline saline, 4 ℃ refrigerator, so repeat, you can achieve the purpose of long-term preservation of species. Compared with other preservation methods, this method is simple, easy to use, etc., it is worth to try.