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目的研究盐酸戊乙奎醚(PHC)对沙土鼠短暂性脑缺血卒中指数的影响,并初步阐明其机制。方法采用沙土鼠双侧颈总动脉阻断模型,分为假手术组、缺血再灌注(模型)组、PHC 0.026、0.24 mg.kg-1组、阿托品组,观察沙土鼠短暂脑缺血再灌注6 h内的神经症状并计算卒中指数;检测海马及皮质谷胱甘肽过氧化物酶(GSH-Px)及Na+,K+-ATP酶的含量;应用流式细胞仪测定海马神经元细胞内游离钙离子的浓度,放射免疫法测定血液中TXB2、6-Keto-PGF1α的含量。结果 PHC能明显降低沙土鼠短暂脑缺血卒中指数,升高海马及皮质Na+,K+-ATP酶及GSH-Px含量,同时减少海马神经元细胞内游离钙离子浓度以及血液中TXB2与6-Keto-PGF1α的比值。结论 PHC可改善沙土鼠短暂脑缺血后卒中的症状,可能与其改善能量代谢、增强清除自由基的能力、减少细胞内游离钙的浓度、降低血液中TXB2/6-Keto-PGF1α的含量有关。
Objective To study the effect of penehyclidine hydrochloride (PHC) on transient cerebral ischemic stroke index in gerbils and to clarify its mechanism. Methods The bilateral common carotid artery occlusion models were divided into sham-operation group, ischemia-reperfusion (PHC) group, PHC 0.026,0.24 mg.kg-1 group and atropine group. The neurological symptoms within 6 h were perfusion and the stroke index was calculated. The content of glutathione peroxidase (GSH-Px) and Na +, K + -ATPase in the hippocampus and cortex were measured. The content of hippocampal neurons was measured by flow cytometry Free calcium ion concentration, radioimmunoassay determination of blood TXB2, 6-Keto-PGF1α content. Results PHC could significantly decrease the short-term cerebral ischemic stroke index, increase the contents of Na +, K + -ATPase and GSH-Px in hippocampus and cortex, and decrease the intracellular free calcium concentration in hippocampal neurons and TXB2 and 6-Keto -PGF1α ratio. Conclusions PHC can improve the symptoms of stroke after transient cerebral ischemia in gerbils, which may be related to improving energy metabolism, enhancing the ability of scavenging free radicals, decreasing the concentration of intracellular free calcium and decreasing the level of TXB2 / 6-Keto-PGF1α in blood.